Cloning and Sequence Analysis of a Thermostable α-Galactosidase Gene of Absidia ramose WL511

doi:10.3969/j.issn.1673-1689.2011.01.022

Home > Archive>Volume 30, Issue 1, 2011 >124-130. DOI:10.3969/j.issn.1673-1689.2011.01.022

Cloning and Sequence Analysis of a Thermostable α-Galactosidase Gene of Absidia ramose WL511
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                        10.3969/j.issn.1673-1689.2011.01.022
                    
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A DNA clone containing α-galactosidase gene fragment(639 bp) was isolated from the constructed cDNA library of Absidia ramose WL511.The 5’-RACE and 3’-RACE techniques confirmed this gene’s upstream and downstream sequences(1817 bp,1092 bp) Sequences analysis revealed that this gene with the full length of 2228 bp made a complete open reading frame of 2142 bp encoding a polypeptide of 713 amino acid residues with predicted molecular mass of 81 kDa and predicted pI of 5.2.This gene(GenBank No.DQ234280),belonging to α-galactosidase 36 family,showed a very low homology with other sources of α-galactosidase genes.The most similarity was 38 % between the α-galactosidases of Streptomyces avermitilis MA-4680 and A.ramose WL511.

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LI He, YOU Juan, GU Qu-liang, LIU Yu-huan. Cloning and Sequence Analysis of a Thermostable α-Galactosidase Gene of Absidia ramose WL511[J]. Journal of Food Science and Biotechnology,2011,30(1):124-130.

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