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Home > Archive>Volume 30, Issue 1, 2011 >144-150. DOI:10.3969/j.issn.1673-1689.2011.01.025
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Screening of Cellulase-Producing Strains from Mangrove Soil and Optimization of Fermentation Conditions
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    Abstract:

    In this study,109 strains with positive signals were collected and screened from mangrove soil using unique C-source plating and differential plating technique for Endo-glucanase,exo-glucanase and β-glucosidase.A fungus HBZ003 with high enzyme activity was obtained identified as Penicillium purpurogenum.The optimum medium components for cellulose production by strain HBZ003 were determined and listed as follows: wheat bran 8 g/L,CMC 2 g/L,(NH4)2SO4 3 g/L,KNO3 2 g/L,KH2PO4 3 g/L,NaCl 6 g/L and CaCl2 0.5 g/L.The maximum activities of carboxymethyl cellulase activity(CMCase) and filter paper activity(FPA) were reached at 16.04U and 4.08 U,respectively,after culture 5 days under 30 ℃,initial pH 4.0 and 160 r/min.

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WANG Chun-yan, LIU Si-xin, GAO Yang, ZHOU Yan-yan, CHEN Tao-tao. Screening of Cellulase-Producing Strains from Mangrove Soil and Optimization of Fermentation Conditions[J]. Journal of Food Science and Biotechnology,2011,30(1):144-150.

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