Abstract:The present study aimed at exploring the effect of Taihe Black-bone silky fowl active peptides and their 13 separated fractions on the proliferation of bone marrow nucleated cells from normal mice.MTT method was used to investigate the effect of active peptides on the proliferation of bone marrow nucleated cells from normal mice.HPLC precolumn derivation with 2,4-dinitrofluorobenzene method was used to determine the amino acid compositions of four active peptide fractions.When mice bone marrow nucleated cells were cultivated for 24 h,it was found that the fractions 7,8,9 and 10 separated from Taihe Black-bone silky fowl active peptides exhibited significantly strong activity(P<0.01) in stimulating mice bone marrow nucleated cells,and the proliferation rate could be up to 130%.Especially,at lower concentration(0.1 μg/mL),fraction 7 was able to significantly stimulate mice bone marrow nucleated cells(P<0.01) and revealed better reproducibility.Fraction 7 was capable of stimulating mice bone marrow nucleated cells proliferation by 298%.Amino acid composition analysis demonstrated that the main amino acids of fraction 7,8,9 and 10 consisted of tyrosine,glycine,glutamic acid,aspartate and alanine.It could be presumed that tyrosine,glycine,glutamic acid,aspartate and alanine may be the material base of active component in stimulating mice bone marrow nucleated cells.
