Establishment of Elispot Assay for Detection of Gentamicin in Milk

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Establishment of Elispot Assay for Detection of Gentamicin in Milk
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This manuscript developt a rapid detection method Elispot assay for Gentamicin sulfate,for this,Anti-GM polyclonal antibody through GM immunogen was obtained;Establishing milk GM-Elispot assay method by spotting coating antigen on PVDF membrane,and the coating antigen and GM in samples competitive binding the site of anti-GM polyclonal,then the HRP catalyzed chromogenic substrate,according to presence or absence of color and depth for interpretation of results.Results: The detection thre-shold of this method was 10 ng/mL,detection time was within 40 minutes and it can achieve semiquantitative detection.The strip of which was stored at 4 ℃ for 90 days could be used and no cross-reaction was found with several structural analogues.The results of samples analysis obtained from the Elispot assay were in a good agreement with that of ELISA method.

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WANG Dan, XU Yang, HE Qing-hua, HUANG Zhi-bing, KANG Min. Establishment of Elispot Assay for Detection of Gentamicin in Milk[J]. Journal of Food Science and Biotechnology,2011,30(2):224-227.

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Online: June 17,2014
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