Cloning,Expression and Characterization of Recombinant Melamine Deaminase in E.coli
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    Abstract:

    The melamine deaminase gene MDA with optimized codon usage was obtained from vector pUC57-MDA,and subcloned into the expression vector 6HisT-pRSET generating recombinant expression vector 6HisT-pRSET-MDA.The recombinant strain BL-MDA was generated after 6HisT-pRSET-MDA was transformed into E.coli BL21(DE3).Expression of BL-MDA was analyzed by SDS-PAGE and the activity of recombinant enzyme was determined by indophenol blue spectrophotometric method.

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TU Zhui, XU Yang, JI Yan-wei, TAO Yong. Cloning, Expression and Characterization of Recombinant Melamine Deaminase in E. coli[J]. Journal of Food Science and Biotechnology,2011,30(2):234-238.

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  • Online: June 17,2014
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