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Cloning and Sequence Analysis of the Exopolysaccharide Biosynthesis Genes from Lactobacillus rhamnosus JAAS8
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    Abstract:

    To clone the RmlA gene in Lactobacillus rhamnosus JAAS8,degenerate primers were designed from the conserved domain of the exopolysaccharide biosynthetic genes of lactic acid bacteria(LAB).The distinctive PCR products with the expected size were amplified from L.rhamnosus JAAS8.The gene sequence(4.1 kb) of RmlACB and Orf1 were cloned by genomic walking method.Nucleotide sequence similarity analyses with GenBank sequences were performed using the BLAST and ClustalX software.GenBank database searching shows that the predicted protein products of the RmlACB genes from L.rhamnosus JAAS8 have highest similarity with the EPS biosynthetic genes of Lactobacillus.The genes RmlA、RmlC and RmlB are closely related to the biosynthesis of dTDP-L-rhamnose precursor.

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DAI Da-hai, LI Sheng-yu, ZHOU Yi-fa, ZHAO Yu-juan, ZHANG Xue, ZENG Xian-peng, YANG Zhen-nai. Cloning and Sequence Analysis of the Exopolysaccharide Biosynthesis Genes from Lactobacillus rhamnosus JAAS8[J]. Journal of Food Science and Biotechnology,2011,30(2):250-254.

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  • Online: June 17,2014
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