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Lactose-Induced Expression of Staphylococcus aureus Enterotoxin A in Escherichia coli BL21(DE3)
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    Abstract:

    Expression of staphylococcus aureus enterotoxin A in Escherichia coli BL21(DE3) using lactose as inducer instead of IPTG was investigated.Effect of inducing conditions,including cell age of recombinant,concentration of lactose,inducing time and adding model of the lactose on the expression level were detaited studied and analyzed.The optimal inducing conditions listed as followed: 0.5 mmol/L(final concentration) lactose by one-time method at the mid-phase of cell growth(OD600=0.6) and then cultured bacteria at 37 ℃,180 r/min for 6 h.The yield of recombinant staphylococcal enterotoxin A induced by lactose was about 36.9% of the total cell solution protein.It was just a little below of that induced by IPTG(38.1%).The price of lactose is only 1% of that of IPTG.All of these suggested that the lactose is available to be effective inducer with lower cost in the process of large-scale production of recombinant protein.

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DING Lan, HOU Xiao-yan, WANG Xiao-hong, CHEN Fu-sheng, ZHANG Yong-xia. Lactose-Induced Expression of Staphylococcus aureus Enterotoxin A in Escherichia coli BL21(DE3)[J]. Journal of Food Science and Biotechnology,2011,30(2):255-260.

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  • Online: June 17,2014
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