Cloning,Expression and Characterization of Clostridium cellulolyticum D-Tagatose 3-Epimerase

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Cloning,Expression and Characterization of Clostridium cellulolyticum D-Tagatose 3-Epimerase
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D-tagatose 3-epimerase(DTE) is the most effective enzyme for the biological production of D-psicose,a novel functional factor.In this study,a novel gene encoding DTE from Clostridium cellulolyticum H10 was cloned.pET-22b(+) was for the plasmid,E.coli BL21(DE3) acted as host cells,then the recombinant strains were constructed.The target protein was over-expressed by IPTG induction;the recombinant DTE purified to electrophoretical homogeneity with affinity chromatography was analyzed by SDS-PAGE,showing approximately 31 000 characteristic protein.The result of activity detection revealed that the recombined enzyme had a higher transforming activity.

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CHU Fei-fei, XING Qing-chao, MU Wan-meng, ZHANG Tao, MIAO Ming, JIANG Bo, ZHOU Liu-ming. Cloning, Expression and Characterization of Clostridium cellulolyticum D-Tagatose 3-Epimerase[J]. Journal of Food Science and Biotechnology,2011,30(2):283-286.

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Online: June 17,2014
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