Determination of Coenzyme NAD~+ and NADH of Saccharomyces cerevisiae Cells in Ethanol Production

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Determination of Coenzyme NAD~+ and NADH of Saccharomyces cerevisiae Cells in Ethanol Production
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Due to the importance of NAD+ and NADH levels in the yeast metabolism,the determination of intracellular NADH/NAD+ is a very interesting for ethanol production.In this study,Three NADH./NAD+ extracting methods,including heating,freeze-thaw cycles,shaking bead,for the disruption and extraction of the NAD+ and NADH in yeast cells were investigated and compared.Finally an efficient process for extraction and detection of NAD+ and NADH in yeast cells was established as follows.Yeast cells were disrupted by shaking bead while NAD+ and NADH were extracted by acid or alkali,then the extracts were detected under the favorable enzymatic cycling system Ⅱ.

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LI Luo-bing, WANG Yong-hong, ZHUANG Ying-ping, CHU Zhu, ZHANG Si-liang. Determination of Coenzyme NAD~+ and NADH of Saccharomyces cerevisiae Cells in Ethanol Production[J]. Journal of Food Science and Biotechnology,2011,30(2):287-294.

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Online: June 17,2014
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