Artificial Antigen Synthesis and Antibody Preparation of Doxycycline

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Artificial Antigen Synthesis and Antibody Preparation of Doxycycline
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Abstract:

In this study,the improved two-step carbodiimide method was used to connecte doxycycline hapten with carrier protein BSA,and doxycycline with the carrier protein OVA to prepare the artificial antigen of doxycycline(BSA-DC) and coating antigen(OVA-DC),respectively.UV scanning analysis and animal immune tests confirm the success in preparation of artificial antigen,and the combination ratio of doxycycline and BSA is 3∶1 by calculating.At the same time,the results showed that high titers of 2.048×106 anti-DC serum were produced by BSA-DC immunized rabbit.It provided a necessary condition for ELISA detecting doxycycline residues.

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GAN Jin-hua, DENG Wei, LI Jin-ping, AI Xiao-hui. Artificial Antigen Synthesis and Antibody Preparation of Doxycycline[J]. Journal of Food Science and Biotechnology,2011,30(2):316-320.

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Online: June 17,2014
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