Expression of Endo-Inulinase gene from Aspergillus ficuum in Escherichia coli

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Expression of Endo-Inulinase gene from Aspergillus ficuum in Escherichia coli
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In order to expand the industrial application of endo-inulinase,its gene from Aspergillus ficuum JNSP5-06 was cloned into the pET-28a(+) and expressed in E.coli BL21(DE3) and get a mutant strain.The mutant was induced by IPTG before the hydrolysis activity was measured and the product was analyzed.The expression vector pET28a-endoI was successfully obtained and the crude enzyme activity was 35 U/mL(fermentation broth).The hydrolysis product from the recombinant inulinase was identified by TLC analysis,indicating that endo-inulinase enzyme solution had activity and the major products were disaccharide,trisaccharide,and tetrasaccharide.These results suggested that the endo-inulinase gene has been successfully expressed in E.coli and significantly improved the endo-inulinase activity.

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CHEN Xiao-ming, CHEN Jing, CHEN Han-qing, JIN Zheng-yu, XU Xue-ming. Expression of Endo-Inulinase gene from Aspergillus ficuum in Escherichia coli[J]. Journal of Food Science and Biotechnology,2011,30(3):388-393.

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