Optimization of Fermentation Conditions for Catalase by Serratia marcescens SYBC08 and Characterization of Its Crude Enzyme

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Optimization of Fermentation Conditions for Catalase by Serratia marcescens SYBC08 and Characterization of Its Crude Enzyme
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The nutrient and environmental conditions for catalase production by Serratia marcescens SYBC08 were optimized with single factor experiment and orthogonal design in this study.The optimum conditions were listed as follows: 25 g/L citric acid,36 g/L corn steep liquor powder,initial pH 6.75,liquid volume 50 mL/250 mL flask,4% inoculation,35 ℃,250 rpm,for 36 hours.With the optimum conditions,the catalase titer achieved at 9553 U/mL,which was 5.49-fold than that of the control.Properties of the catalase after purification by ammonium sulfate precipitation were studied.At 60 ℃ and pH 9.0,the enzyme was stable for 150 min;at 65 ℃ and pH 9,the half-life of the enzyme was approximately 150 min;its thermo stability was higher than that of commercial catalase from bovine.It was also active at 20 ℃ and had 78% of its activity at 0 ℃.These results suggest that the enzyme displays a property with cold adaption and thermo stability,and it has potential applications in high-temperature,alkaline and low-temperature conditions.

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ZENG Hua-wei, ZHANG Feng, CAI Yu-jie, LIAO Xiang-ru, LI Jiao-yang, XING Yu-peng, ZHANG Da-bin. Optimization of Fermentation Conditions for Catalase by Serratia marcescens SYBC08 and Characterization of Its Crude Enzyme[J]. Journal of Food Science and Biotechnology,2011,30(3):410-416.

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