Determination of Content of Coenzyme Q10 in Copolymer Micellers with SDS Solubilization

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Determination of Content of Coenzyme Q10 in Copolymer Micellers with SDS Solubilization
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The total content of nutrient is an important index for evaluating the quality of casein-dextran copolymer micellers as a delivery system.Sodium dodecyl sulfate(SDS) as a surfactant can be used to disorganize copolymer micellers and make coenzyme Q10 solubilize in water for quantitative analysis.In this study,the recovery rate of coenzyme Q10 from blank casein-dextran copolymer micellers ranged from(98.77±1.60) % to(95.90±0.80) % with conditions as follows: SDS concentration 0.31 mol/L,incubation temperature 30 ℃,sodium borohydride(7 mg/mL) dL,reduction time 3~5 min.The analysis of synchronous and exogenous fluorescence spectra indicated that the hydrophobic region of casein unit of copolymer was partly changed during solubilization at SDS concentration form 0 to 0.5 mol/L.The interaction between SDS and hydrophobic region was not influenced by temperature when the concentration of SDS was 0.31 mol/L.

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LI Li, XIA Shu-qin, LI Xue-qi, ZHANG Xiao-ming, JIA Cheng-sheng, YUE Lin. Determination of Content of Coenzyme Q10 in Copolymer Micellers with SDS Solubilization[J]. Journal of Food Science and Biotechnology,2011,30(4):511-517.

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