Protoplast Fusion of L-Valine Producer and Screening of Fusants by Improving High-Glucose Tolerance

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Protoplast Fusion of L-Valine Producer and Screening of Fusants by Improving High-Glucose Tolerance
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A fusant which could be resistant to high concentration of glucose was screened by fusing protoplasts in this study.The L-valine overproducing fusant was derived from Brevibacterium flavum NV128 and JV16(Leu-Ile-Met-),and was screened on high-glucose gradient plates by means of fusing the single inactivated protoplasts and diethylsulfate(DES) treatment.At the same time,three different conditions(penicillin,lysozyme and osmotic stabilizer)affecting the formation and regeneration of protoplasts were studied and the optimum conditions were determined.Under the optimum conditions,the titer of L-valine achieved at 46.8g/L after 72h culture,and the yield of L-valine on glucose was 27.7%.

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YUAN Dan-dan, ZHANG Wei-guo. Protoplast Fusion of L-Valine Producer and Screening of Fusants by Improving High-Glucose Tolerance[J]. Journal of Food Science and Biotechnology,2011,30(4):597-601.

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