Study on Chemical Modification of Polygalacturonase with Chitosan Oligosaccharides

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Study on Chemical Modification of Polygalacturonase with Chitosan Oligosaccharides
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Purified polygalacturonase(PG) was chemically modified with water soluble chitosan oligosaccharide(COS) as modifier.The optimum modification conditions were determined by the single factor tests and orthogonal tests and listed as follows: purified PG 80 mg,pH 4.0,temperature 3 ℃,reaction time 12 h,COS content 150mg.Under the optimum conditions,The specific activity of the modified PG was 340.10 U/mg protein,higher 153.42% than that of the unmodified PG.

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LIU Xiao-lin. Study on Chemical Modification of Polygalacturonase with Chitosan Oligosaccharides[J]. Journal of Food Science and Biotechnology,2011,30(6):812-817.

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Online: June 17,2014
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