Identification of a Bacterium Isolated from the Decayed Cyanobacteria and Its Fermentation for Protease Production

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Identification of a Bacterium Isolated from the Decayed Cyanobacteria and Its Fermentation for Protease Production
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Morphological aspects,physiological and biochemical characters and 16 S rRNA sequence were utilized to identify the bacterium isolated from the decayed cyanobacteria in this study,and liquid state fermentation was utilized to evaluate the effect of carbon and nitrogen resources on its protease production.The data showed that the bacterium isolated was belonged to the genus Serratia designated as Serratia marcescens SYBC YH.The optimal carbon resource was fructose,glucose,feather powder,or powder of corn seed.The optimal nitrogen resource was corn steep powder,yeast extract or beef extract.The strain could produce protease by liquid state fermentation with cyanobacteria as sole nitrogen resource to produce organic solvent tolerant protease.The optimal catalytic temperature and pH was 40℃ and 7.0 respectively.

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YIN Jing, CAI Yu-jie, LIAO Xiang-ru, HAO Zhi-kui, LI Ting-ting, LI Gui-ying, ZHANG Da-bing. Identification of a Bacterium Isolated from the Decayed Cyanobacteria and Its Fermentation for Protease Production[J]. Journal of Food Science and Biotechnology,2011,30(6):911-916.

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Online: June 17,2014
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