Isolation and Identification of a Strain with Producing Bone Collagenase

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Isolation and Identification of a Strain with Producing Bone Collagenase
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In this study,a novel strain MBL13 was isolated from screened the soil in the animal bone factories through screening methods.It was found that the strain could secreted 36.80±1.93 U/mL collagenase in fermentation broth.Furthermore,the strain was identified as Bacillus cereus through morphology,physiology biochemistry characteristic,16S rDNA sequencing and Fourier Transform Infrared determination.the collagenase secreted from MBL13 exhibited higher hydrolysis capacity on bovine bone collagen than that of type I collagenase(United States).It was believed that the collagenase from MBL13 has the specific restriction sites due to the bands of peptides generated after digestion was not continuous.

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LIU Li-li, YANG Xie-li. Isolation and Identification of a Strain with Producing Bone Collagenase[J]. Journal of Food Science and Biotechnology,2011,30(6):917-923.

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