Preparation of Polyclonal Antibodies of Serpin and Preliminary Screening of the Bifidobacteria Species of Yielding Serpin

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Preparation of Polyclonal Antibodies of Serpin and Preliminary Screening of the Bifidobacteria Species of Yielding Serpin
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Serpin was amplified from the genome of Bifidobacterium longum WBL001.Recombinant plasmid pBX2-serpin was constructed and expressed in Escherichia coli for Serpin production.The recombinant protein was purified and used as antigen for mice immunization.Antiserum was purified specifically by Serpin-coupled magnetic beads.The antibody eluting was optimized by using different eluents.Serpin-like protein was screened out from 11 Bifidobacteria strains by colony immunoblotting.The result indicated that 1 mol/L NaOH is the best fluent for antibody elution from magnetic beads(about 50% recovery).Non-specific reactions of antiserum with lactic acid bacterium and pathogens were eliminated after purification.Serpin-like protein had been found in B.infantis WBAN07 from 11 Bifidobacteria strains by colony immunoblotting.

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CUI Jia, WAN Cui-xiang, YANG You-jun, LI Peng, WEI Hua, ZHANG Zhao-lin, XU Di, XU Feng, LI Bo. Preparation of Polyclonal Antibodies of Serpin and Preliminary Screening of the Bifidobacteria Species of Yielding Serpin[J]. Journal of Food Science and Biotechnology,2011,30(6):934-939.

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