Abstract:In this manuscript,effect of ethanol solutions on the biochemical property and conformation of cutinase were studied.The results showed that the optimum temperatures of cutinase were 65 ℃,55 ℃ and 50 ℃ respectively in 20%(V/V),30% and 40% ethanol solutions.In 20% and 50% ethanol solutions cutinase was stable at 37℃.In 30% and 50% ethanol solutions the optimum pH of cutinase were 9.5 and 9.0 respectively.In 30% ethanol solution the pH stability range of cutinase was 6.5-10.5.In 30% and 40% ethanol solutions activity of cutinase at 37 ℃was enhanced.The inactivation kinetic of cutinase was researched in ethanol solutions at 50 ℃.The results showed that the inactivation kinetic of cutinase in ethanol solutions followed the first order reaction(R2>0.98).And the secondary and the tertiary structure conformations greatly changed in ethanol solutiods.
