Determination of the Content of Strychnine and Brucine in Strychnos nux-vomica L.by the Liquid Fermentation with Trametes cinnabarina(Jacq.) Franeh

doi:10.3969/j.issn.1673-1689.2012.03.012

Home > Archive>Volume 31, Issue 3, 2012 >294-301. DOI:10.3969/j.issn.1673-1689.2012.03.012

Determination of the Content of Strychnine and Brucine in Strychnos nux-vomica L.by the Liquid Fermentation with Trametes cinnabarina(Jacq.) Franeh
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                        10.3969/j.issn.1673-1689.2012.03.012
                    
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Based on the results of the single factor(carbon and nitrogen sources),the effect of broth content,mycelial pellet amount,powder weight and fermentation time on alkaloids content and biomass growth were further discussed by using L16(45) orthogonal test.The optimized culture conditions were acquired and listed as follows: maltose is carbon source,soybean cake powder is nitrogen source and liquid volume of 100 mL(a 250 mL flask),by adding 0.25 g powder,inoculated three evenly sized mycelial pellet,cultured at 28 degrees celsius for 5 days.After optimization the total content of strychnine and brucine in the culture medium reduced 8.74% compared with the control.Biomass could reach 10.6 g/L.Its rising rate was 37.7%.Meanwhile strychnine N-oxide and brucine N-oxide increased.The results showed the drug could promote the fungal growth and it can decrease the content of toxic constituents by liquid fermentation.

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LIU Xue-xiang, PAN Yang, JIANG Ya-ping, WEI Qing-fang. Determination of the Content of Strychnine and Brucine in Strychnos nux-vomica L. by the Liquid Fermentation with Trametes cinnabarina(Jacq.) Franeh[J]. Journal of Food Science and Biotechnology,2012,31(3):294-301.

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