Abstract:Purpose: To investigate the separation method and the structure of polysaccharides with hypoglycemic effect from barley malt(BP).Methods: Alloxan-induced diabetic mice model was built,in which the hypoglycemic effect of polysaccharides from barley malt were determined.The polysaccharides from barley malt was purified to homogeneity through DEAE-cellulose,HW-55F and Sephacryl S-400 column chromatography.Meanwhile,the purity identification was analyzed by phenol-sulfuric method and thin layer chromatography.The structure was investigated using sugar composition analysis and 1H-NMR,13C-NMR,135DEPT-NMR analysis.Results: The polysaccharides can decrease fasting plasma glucose of the diabetes mice efficiently,and showed no significant effect on postprandial plasma glucose.Neutral polysaccharides(NBP)and acidic polysaccharides was isolated by DEAE-cellulose.BPⅠ and BPⅡ were further isolated from NBP by HW-55F and Sephacryl S-400.BPⅠconsists only of glucofuranose units joined by 1→3 bond,and both of α and β-configuration appear alternately.The main chain of BPⅡ is galactoses linked by 1→4 bond,and the galactose,forming lateral chain,is linked to C-6 of galactose in the main chain.BPⅡ is reported for the first time from barley malt.
