Preparation of High-Activity Direct Vat Set Starter of Lactobacillus casei with Novel Cryoprotactants

doi:10.3969/j.issn.1673-1689.2012.11.002

Home > Archive>Volume 31, Issue 11, 2012 >1128-1135. DOI:10.3969/j.issn.1673-1689.2012.11.002

Preparation of High-Activity Direct Vat Set Starter of Lactobacillus casei with Novel Cryoprotactants
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                        10.3969/j.issn.1673-1689.2012.11.002
                    
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To conquer the survival decline of direct vat set(DVS) starter during freeze-drying process,Lactobacillus casei Zhang was used to investigate the effect of different cryoprotectants on physiological activity of cells.The results showed that with the participation of traditional freeze-drying protective agents such as skimmed milk and sorbitol,the survival rate of freeze-dried cells improved to 54.5% by adding 5 g/L glutathione(GSH),and the number of viable cells increased to 6.16 ×10 8 cfu/mL.Further analysis indicated that GSH improved the instauration of cell membrane fatty acids(U/S value) after freeze-drying treatment by 2.02 times compared with the control sample,while it reduced the chain length of saturated fatty acids from 17.1 to 16.0.In addition,the result of transmission electron microscope(TEM) suggested that GSH is able to maintain the integrity of cell membrane structure effectively in freeze-drying process.All of these results provide a constructive way to find novel cryoprotactants based on the understanding of the physiological changes of microbial cells during the freeze-drying process.

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ZHANG Juan, LIU Qian, WU Chong-de, DU Guo-cheng, CHEN Jian. Preparation of High-Activity Direct Vat Set Starter of Lactobacillus casei with Novel Cryoprotactants[J]. Journal of Food Science and Biotechnology,2012,31(11):1128-1135.

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