Abstract:Gynostemma pentaphyllum is known as "Southern Ginseng".It contains triterpenoid saponins of the dammarane-type structure with the same skeleton of ginsenosides.In this manuscript,the saponin components of Gynostemma pentaphyllum were analyzed by HPLC,which choosed the concentration of ethanol extraction,and it was found that that adopted to the extract gypenosides(GYP) was from 75 % ethanol,polar gypenosides(GYP1) from 45% ethanol,and lower polar gypenosides(GYP 2) from 90% ethanol.Furthermore,the optimum extraction parameters for GYP 1 and GYP 2 were studied by Orthogonal method and listed as follows: GYP 1 was extracted with thirty fold-weight 45 % enthanol in 70 ℃ water bath for 12 h,and GYP 2 was extracted with thirty fold-weight 90% ethanol in 60 ℃ water bath for 6 h.Anti-hepatoma activity was determined tests by MTT and the results showed that GYP and GYP 2 can inhibit hepatoma cell SMCC7721 with IC 50 value 165.83 ±14.12 μg/mL and 113.97 ±9.26 μg/mL,respectively.However,GYP 1 without any anticancer activity.The result showed that Gynostemma pentaphyllum exhibited the anti-hepatoma activity caused by lower polar saponins.
