Analysis of Conjugated Linoleic Acid Isomers by Gas Chromatography

doi:10.3969/j.issn.1673-1689.2012.11.018

Home > Archive>Volume 31, Issue 11, 2012 >1226-1231. DOI:10.3969/j.issn.1673-1689.2012.11.018

Analysis of Conjugated Linoleic Acid Isomers by Gas Chromatography
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                        10.3969/j.issn.1673-1689.2012.11.018
                    
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Abstract:

Conjugated linoleic acids(CLA) are the positional and geometric isomers of linoleic acid,among them,9 c,11t-CLA and 10 t,12 c-CLA exhibited the stronger biological activity.In this study,a method was developed for the quantitative determination of 9 c,11t-CLA and 10 t,12 c-CLA by capillary gas chromatography.The sample of CLA oil was methylated with KOH in methanol.The calculation of results was carried out with external standards.The recovery of 9c,11tCLA and,10 t,12 c-CLA was between 97.66% ～103.51%,96.12% ～102.48%,respectively.The detection and quantitation limits were separately 2.4 mg/kg and 8 mg/kg.

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WANG Wei, YANG Hong, YANG Tian-kui. Analysis of Conjugated Linoleic Acid Isomers by Gas Chromatography[J]. Journal of Food Science and Biotechnology,2012,31(11):1226-1231.

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Online: June 17,2014
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