Detecting the Pectin in the Tobacco Stems after Enzymatic by Combing the Gravimetric Method with HPLC

doi:10.3969/j.issn.1673-1689.2012.12.015

Home > Archive>Volume 31, Issue 12, 2012 >1325-1330. DOI:10.3969/j.issn.1673-1689.2012.12.015

Detecting the Pectin in the Tobacco Stems after Enzymatic by Combing the Gravimetric Method with HPLC
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                        10.3969/j.issn.1673-1689.2012.12.015
                    
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It combined the gravimetric method with HPLC to detect different kinds of pectin in the tobacco stems and cut-stems after enzymatic.Firstly,the sample of tobacco stems and cut-stems after enzymatic should be treated by acid hydrolysis and filteration.Then we detected the content of calcium pectinate in the solid samples by gravimetric method.The galacturonic acid in the supernatant that was discarded in the process of sample treatment will be tested by HPLC.These two parts of the content of pectin could be conversed and added together.The analytical results showed that the coefficient of variation was 0.33%,the average recovery of galacturonic acid was 99.32%.Using this method,the coefficient of variation of the content of the total pectin acid was lower than traditional gravimetric method.The pectin would be fully extracted.The detection method is comprehensive and with good reproducibility,and easy operations.

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SHI Lin-yan, XU Gan-rong, JIN Yong-ming, TANG Zhao-qi. Detecting the Pectin in the Tobacco Stems after Enzymatic by Combing the Gravimetric Method with HPLC[J]. Journal of Food Science and Biotechnology,2012,31(12):1325-1330.

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Online: June 17,2014
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