Screening and Identification of Lipase Inhibitor-Producing Strain

doi:10.3969/j.issn.1673-1689.2013.02.018

Home > Archive>Volume 32, Issue 2, 2013 >219-223. DOI:10.3969/j.issn.1673-1689.2013.02.018

Screening and Identification of Lipase Inhibitor-Producing Strain
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                        10.3969/j.issn.1673-1689.2013.02.018
                    
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We collected many samples from different locations,then isolated and purified single colony. By fermentation,the products whether have lipase inhibition activity as a screening indicator. We find a good strain Sf16 after we cultured 2013 wild strains. The morphological characteristics of colony,aerial hyphae,sporothrix and spores and the growth performance characteristics in different carbon sources medium of strain Sf16,as well as some physiological and biochemical characteristics were observed. Extract the DNA,amplified and determined the 16SrDNA.Then homology search in database,compared and constructed the phylogenetic tree. Inhibitor-producing strain Sf16 preliminary identification by means of morphological and molecular biology techniques,the result is Streptomyces sp.

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LIU Lan, ZHOU Pei-hua, ZENG Wei, CHEN Gui-guang, LIANG Zhi-qun. Screening and Identification of Lipase Inhibitor-Producing Strain[J]. Journal of Food Science and Biotechnology,2013,32(2):219-223.

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Online: June 17,2014
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