Optimization of Two-Dimensional Electrophoresis Conditions for Proteome Analysis of Peach Fruit

doi:10.3969/j.issn.1673-1689.2013.03.004

Home > Archive>Volume 32, Issue 3, 2013 >250-257. DOI:10.3969/j.issn.1673-1689.2013.03.004

Optimization of Two-Dimensional Electrophoresis Conditions for Proteome Analysis of Peach Fruit
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                        10.3969/j.issn.1673-1689.2013.03.004
                    
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An effective two-dimensional electrophoresis system was developed to determine the total protein from "huiyulu" peach fruit in this study.The optimum conditions for total protein detection were listed as follows: phenol extraction,800 μg protein sample,17 cm IPG strip with pH 4-7,and finally detecting proteins with modified colloidal coomassie brilliant blue G-250 staining.With the optimum conditions,a high quality 2-DE map with low background was achieved.

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ZHANG Li, JIANG Li, SHI Yun, KANG Ruo-Yi, WANG Li-bin, YU Zhi-Fang. Optimization of Two-Dimensional Electrophoresis Conditions for Proteome Analysis of Peach Fruit[J]. Journal of Food Science and Biotechnology,2013,32(3):250-257.

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Online: June 17,2014
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