Abstract:The extraction parameters of aminopeptidase by Ultra-flo system from the fermentation broth is studied in this manuscript.The optimum conditions for high high enzyme activity recovery and membrane flux were listed as follows: 30 kDa MWCO polyether sulfone(PES) membrane,the operation pressure between 0.2 MPa and 0.25 MPa,the initial pH value 5.5～6.5 and the concentration ratiof 3 times;The elution is in the form of regular flow of water,and the elution volume is about 10 times of the concentration of the fermentation broth.By the optimun conditions,the enzyme activity recovery rate was achieved at 85% and 79% of the desalination process,and the salt in the concentration of the fermentation broth has been removed about 90%,and the final concentration factor can be controlled for 8～10times.The enzyme activity recovery rate can be 67.15% of the whole of the Ultrafiltration process,and the membrane flux can be maintained above 16 L/(m2·h) throughout the process.
