Method of Nisin Detection in Food by Enzyme-Linked Immunosorbent Assay

doi:10.3969/j.issn.1673-1689.2013.05.010

Home > Archive>Volume 32, Issue 5, 2013 >509-515. DOI:10.3969/j.issn.1673-1689.2013.05.010

Method of Nisin Detection in Food by Enzyme-Linked Immunosorbent Assay
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                        10.3969/j.issn.1673-1689.2013.05.010
                    
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Abstract:

The aim of this study was to develop a rapid quantitative melamine ELISA test kit for the detection of Nisin in dairy,meat and milk drink.The indirect competitive enzyme-linked immunosorbent assay was established based on the Nisin specific polyclonal antibody.The sensitivity was 0.1 μg/mL,the standard curve range was 0.1～62.5 μg/mL,the detection limit of the dairy and meat were 1.0 μg/mL,and 0.5 μg/g,respectively.The average recoveries of milk drink and meat ranges between 80% ～120%,and the coefficient of variation was <20%.The indirect competitive ELISA has been proven to be rapid,sensitive,and simple.It is suitable for the detection of the Nisin content in dairy,meat and milk drink.

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MENG Ru, ZHAO Gui-ming, JIA Yu, WANG Zhen-bao. Method of Nisin Detection in Food by Enzyme-Linked Immunosorbent Assay[J]. Journal of Food Science and Biotechnology,2013,32(5):509-515.

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Online: June 17,2014
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