Construction of Pichia stipitis Genomic Library and Cellobiase Gene Screening

doi:10.3969/j.issn.1673-1689.2013.05.014

Home > Archive>Volume 32, Issue 5, 2013 >536-540. DOI:10.3969/j.issn.1673-1689.2013.05.014

Construction of Pichia stipitis Genomic Library and Cellobiase Gene Screening
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                        10.3969/j.issn.1673-1689.2013.05.014
                    
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In this study,a genomic library for Pichia stipitis was firstly constructed.The library covered 1.08 times of the15 441 179bp genomic DNA of the strain,and contained 3 000 clones with the insertion sizes ranging from 0.5 to 8.0 Kb.It has a propability of 97% for screening a random gene form this library.The library plasmids were extracted and transformed into Saccharomyces cerevisiae W303-1A by LiAc method.At last,a 1.82 Kb cellobiase gene of P.stipitis was firstly characterized by means of screening the transformants on cellobiose plates.

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MA Jing-wei, ZHANG Liang, XUE Wei, SHI Gui-yang. Construction of Pichia stipitis Genomic Library and Cellobiase Gene Screening[J]. Journal of Food Science and Biotechnology,2013,32(5):536-540.

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Online: June 17,2014
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