Establishment of Two-Dimensional Electrophoresis Conditions for Proteomic Analysis of Zizania latifolia

doi:10.3969/j.issn.1673-1689.2013.06.004

Home > Archive>Volume 32, Issue 6, 2013 >581-585. DOI:10.3969/j.issn.1673-1689.2013.06.004

Establishment of Two-Dimensional Electrophoresis Conditions for Proteomic Analysis of Zizania latifolia
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                        10.3969/j.issn.1673-1689.2013.06.004
                    
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In order to establish the two-dimensional electrophoresis(2-DE) conditions of Zizania latifolia,the effects of different protein extraction methods,loading amount,pH ranges of IPG strip and gel concentration,etc.on 2-DE maps were investigated.The results showed that the modified phenol extraction protocol is more suitable for total protein extraction of Z.latifolia compared with TCA/acetone protocol.And a high quality 2-DE map with more protein spots,clear background and high protein point resolution was obtained using the following optimized procedure:loading 1.0mg protein sample on 17 cm IPG strip with pH 4-7,SDS-PAGE with 12 g/dL gel concentration,and finally detecting proteins with coomassie brilliant blue G250 staining.The research provides a basis for further study of the Z.latifolia proteomics.

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LUO Hai-bo, CHEN Wei, ZHOU Jing-feng, SONG Hui-bo, YU Zhi-fang. Establishment of Two-Dimensional Electrophoresis Conditions for Proteomic Analysis of Zizania latifolia[J]. Journal of Food Science and Biotechnology,2013,32(6):581-585.

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Online: June 17,2014
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