Determination of Dapsone Residue in Animal-Originated Food by Enzyme-Linked Immunosorbent Assay
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    Abstract:

    A rapid detection method for dapsone residue in animal-originated food was established based on indirect competitive enzyme-linked immunosorbent assay.The results showed that the 50% inhibitory concentration(IC50) of the method was 0.369 μg/L.The detection limit for chicken and pork was 0.2 μg/kg,for egg and honey was 2 μg/kg,and for milk was 0.6 μg/L.Sample recoveries were 78.2%~104.0%,and the coefficients of variation were 5.2%~13.5%.Results of the method were in accordance with those of liquid chromatography-tandem mass spectrometry(LC-MS/ MS).This method has the advantage of being sensitive and accurate,and suitable for the rapid detection of dapsone residue in animal-originated food.

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TAO Guang-can, LI Yong, CUI Ting-ting, WANG Jiao, FU Sheng. Determination of Dapsone Residue in Animal-Originated Food by Enzyme-Linked Immunosorbent Assay[J]. Journal of Food Science and Biotechnology,2013,32(7):778-783.

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  • Online: June 17,2014
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