Extraction of Water-Soluble β-carotene from Carrots by Enzymatic Hydrolysis

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Extraction of Water-Soluble β-carotene from Carrots by Enzymatic Hydrolysis
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Abstract:

The key technical parameters by enzymatic hydrolysis to extract water-soluble β-carotene from carrots were investigated.The optimal technological process determined by single factor experiment was as follow:50 g fresh carrot was homogenated,and then 100 mL 0.2 g/dL Tween-80,1.5 g cellulase and 0.4 g pectinase were added;the carrot pulp was smashed for 40 min by ultrasonic wave,then pH was adjusted to 6.0;after stirring for 2 h at 40 ℃,2 g diatomite was added and finally filtrated.The optimal factors obtained from response surface analysis were 0.49 g pectinase,enzymatic hydrolysis pH was 6.08 and ultrasonic time was 41.58 min.Under above conditions,the extraction yield for water soluble β-carotene reached 12.23 μg/g.

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ZHANG Wei-wei, WU Yan-wen, OUYANG Jie. Extraction of Water-Soluble β-carotene from Carrots by Enzymatic Hydrolysis[J]. Journal of Food Science and Biotechnology,2013,32(8):854-860.

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Received:December 03,2012
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Online: October 28,2013
Published: August 25,2013

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