Screening, Identification and Transformational Property of Minor Ginsenoside Compound K-Producing Fungus

doi:10.3969/j.issn.1673-1689.2013.09.003

Home > Archive>Volume 32, Issue 9, 2013 >914-920. DOI:10.3969/j.issn.1673-1689.2013.09.003

Screening, Identification and Transformational Property of Minor Ginsenoside Compound K-Producing Fungus
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                        10.3969/j.issn.1673-1689.2013.09.003
                    
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Ten fungi were screened with regard to their ability to convert major saponins Rb1,Rb2and Rc to Compound K(C-K) which has notable anti-tumor activity. Among these,strain sp. 3.26exhibited highest transfer ability. The strain sp. 3.26 was identified as Epicoccum sp. through ITS sequence analysis and morphology observation. A crude ginsenoside-hydrolyzing β-glucosidase(EI) was isolated from a culture filtrate of the strain sp. 3.26. The optimal pH and temperature of E-I were pH 5.0 and 40 ℃,respectively. E-I not only hydrolyze ginsenoside Rb1but also hydrolyze Rb2and Rc. However,E-I has higher activity to Rb1. This result indicated that the glycosidases secreted by strain sp. 3.26 has wide substrate specificity,and was more specific to glucosidic linkage,and the biotransformation pathways by this strain was:ginsenosides Rb1,Rb2,Rc→Rd→F2→C-K.

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ZHAO Xue-song, LIANG Fei, HU Yan-bo, MENG Yue, ZHOU Yi-fa. Screening, Identification and Transformational Property of Minor Ginsenoside Compound K-Producing Fungus[J]. Journal of Food Science and Biotechnology,2013,32(9):914-920.

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