A Fast, Efficient and Economical Method for Isolating Plasmid From Yeast

doi:10.3969/j.issn.1673-1689.2013.11.012

Home > Archive>Volume 32, Issue 11, 2013 >1194-1198. DOI:10.3969/j.issn.1673-1689.2013.11.012

A Fast, Efficient and Economical Method for Isolating Plasmid From Yeast
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                        10.3969/j.issn.1673-1689.2013.11.012
                    
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Plasmid extraction is an important but difficult step of the genetic engineering operations,because of the yeasts cells with tough and unbreakable cell wall. For getting one fast,simple,efficient and stable method of plasmid extraction from yeasts,we employed the techniques of agarose gel electrophoresis,spectrophotometer and PCR amplification,and compared the yield,purity of plasmids which were isolated from yeasts with the methods of glass beads-liquid nitrogen,snailase and kit. The results showed that the method of glass beads-liquid nitrogen had the advantages of high efficiency,good quality,low cost,simple operation in contrast to the other two methods,and it can be used as a laboratory scale commonly method for extraction of yeast plasmid.

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ZHANG YI, LIU Kun, CAO Peng, WEI Sen, WEI Dan-dan, DU Jian, LI Peng-kun, LI Cheng-wei. A Fast, Efficient and Economical Method for Isolating Plasmid From Yeast[J]. Journal of Food Science and Biotechnology,2013,32(11):1194-1198.

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Online: June 17,2014
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