Construction and Optimization of Proteomic Map of Saccharomyces cerevisiae

doi:10.3969/j.issn.1673-1689.2014.03.002

Home > Archive>Volume 33, Issue 3, 2014 >235-240. DOI:10.3969/j.issn.1673-1689.2014.03.002

Construction and Optimization of Proteomic Map of Saccharomyces cerevisiae
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                        10.3969/j.issn.1673-1689.2014.03.002
                    
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Total proteins of Saccharomyces cerevisiae was extracted and separated by two-dimensional electrophoresis(2-DE) in this study. Different sample preparation methods,different parameters of the gel and different staining methods were compared for the optimization of 2-DE. Finally,2D-gel without obvious vertical and horizontal stripes,with low background,high resolution and good reproducibility was obtained. Analyzed by the PD-Quest software,about 1 000 protein spots were isolated from the 2-DE protein map. The construction of 2-DE protein map provide a firm basis for the follow-up nitrogen metabolism study.

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ZHAO Shaohui, ZHOU Jingwen, DU Guocheng, CHEN Jian. Construction and Optimization of Proteomic Map of Saccharomyces cerevisiae[J]. Journal of Food Science and Biotechnology,2014,33(3):235-240.

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Online: June 16,2014
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