Abstract:Ovalbumin hydrolyzate was prepared by pepsin and trypsin hydrolysis. Two hydrolyzate fractions with antibacterial activity F21 and F22,were separated by A咬KTA protein purification systems,dialysis,and RP-HPLC. The effects of F21 and F22 on cell membrane permeability and membrane integrity and their DPPH o radical scavenging ability were also investigated. The inhibition rates of 50 μg/mL of F21 and F22 on Escherichia coli were 84.6% and 22.5%respectively,and the inhibition rates on Staphylococcus aureus were 53.3% and 29.6%.Galactosidase release and flow cytometry analysis showed that the two fractions could destroy Escherichia coli cell membrane permeability and membrane integrity. In addition,they could effectively scavenge DPPH o radical. When the concentrations were 2.0 mg/mL,the DPPH o radical scavenging abilities of the two fractions were close to the scavenging ability of 1.0 mg/mL GSH. As a result,ovalbumin hydrolysate could be used as antibacterial agents and antioxidants in further exploitation.
