Abstract:Making use of a definite molecular weight polyacylic acid sodium as dispersant to purify superoxide dismutase from blood,and to explore the effect of different molecular weight of PAAS on SOD activity of blood,in consideration of the purification materials of the ion exchange chromatography and gel filtration chromatography was expensive,so as to explore a suitable method of the SOD purification for the industrialized mass production.Making use of cheap materials such as:chitosan,IDA and epichlorohydrin to synthesis a new type of filler,according to the principle of chelating chromatography,raw protein was removed and electrophoresis pure level superoxide dismutase was obtained,the enzyme activity was further improved,the self-made materials can take place of the tradition materials such as Sephedex DEAE-sepherose-fast flow.The results of the SDS-PAGE of the crud enzyme after ultrafiltration by ion exchange chromatography showed a single band obviously,and reached the electrophoresis purity,the enzyme activity is up to 5 585 u/mg, 6 148 u/mg,4 739 u/mg,and the product yield is 18.1%,13.7%,15.5% respectively,active recovrery is 61.2%,50.8%,42.6% resectively after purification.The low molecular weight polyacrylic acid is more suitable in the Purification process of blood is suitable as a polymer electrolyte to purify the blood SOD,the medium and high molecular weight PAAS are not appropriate for industrialized purification application.
