Abstract:Sialyl lewis x(Slex) was one of the common glyco ligands of the selectin family. Slex combining with the inflammatory cells competitively could make inflammation reaction restrain efficiently. Clone and expression of the key enzymes in the biosynthesis process of Slex made the biosynthesis process of Slex in vitro possible,and it was also good for some related research of biological agents. Alpha-(1,3)-fucosyltransferase(FT) was the very one of the key enzymes in the biosynthesis process of Slex. In order to understand some related physicochemical properties of the FT gene,its sequence was analyzed by using approaches of bioinformatics online. The FT gene was inserted into a recombinant plasmid pMD19-T,and subcloned to the expression plasmid pPIC9K later. The linear expression plasmid pPIC9K-GT was integrated to the genome of Pichia pastoris GS115 by electrotransformation. After inducible expression,the soluble target protein was detected by SDS-PAGE. The result demonstrated that successfully expressed in P. pastoris GS115.
