Abstract:with the Rhodamine B content in extract as index, the optimum parameters,including dispersant type,the ratio of dispersing agent and sample,composition of the eluent and elute volume,of extracting Rhodamine B from chili were investigated by using orthogonal design L16(45).The optimum conditions for extracting Rhodamine B from chili powder are as follows:dispersant type is silica gel,the ratio of dispersing agent and sample is 1∶1,composition of the eluent is 80% of acetonitrile,elute volume is 20 mL. The optimum conditions for extracting Rhodamine B from pepper oil are as follows:dispersant type is neutral aluminum oxide,the ratio of dispersing agent and sample is 2 ∶1,composition of the eluent is 80% of methanol,elute volume is 20 mL. The optimum conditions for extracting Rhodamine B from chili paste are as follows:dispersant type is neutral aluminum oxide,the ratio of dispersing agent and sample is 3∶1,composition of the eluent is80% of acetonitrile,elute volume is 5 mL. The extraction method is simple,convenient,less material consumption and less organic solvent.
