Abstract:The process parameters for the microwave-assisted extraction of cAMP from Hetianyuzao systemically was investigated in the study,and the results were compared with the traditional solvent extraction and ultrasonic-assisted extraction methods.The processing parameters of extracting cAMP from Hetian jade jujube with microwave-assisted extraction were obtained and listed as follows that:ethanol concentration 10%,microwave-assisted power 300 W,microwave-assisted extraction time3min,soak period 6 h,liquid/solid ratio 1 ∶30;Under the optimal extraction conditions,the yield of cAMP was 795.63 μg/(g·dw). The effect sequence of four main factors for the yield were as follows: microwave time>microwave power>liquid/solid ratio>soak period time. Microwave-assisted method extracted more cAMP than that of the traditional solvent extraction during short time,and extracted more cAMP than ultrasonic wave-assisted extraction with low energy consumption. The separation efficiency of cAMP by LS-200 was 97.81%,in 3.47% purity.
