Abstract:The method of simultaneous determination of five kinds of artificial sweetener neotame, aspartame,sodium cyclamate,sodium saccharin,acesulfame content was established by HPCE with UV-Vis detector in this manuscript. The fused silica capillary with uncoated inner wall(50 μm×75 cm,effective length 60 cm) was used,and the capillary was kept at 25 ℃. 0.2 mol/L borate(pH= 8.29) was adopted as electrophoresis buffer at +23 kV separation voltage. The sample was injected under 3.45 kPa pressure for 3.0 seconds,and detection wavelength being set at 200 nm. The linear regression equation correlation coefficient r for five kinds of sweeteners was between 0.999 21 and 0.999 89. The detection limit of five kinds of sweeteners was in the range of 2.2~552.6 mg/L. The spiked recoveries were within the range from 96.29% to 102.31%,and the relative standard deviation was kept between 3.81% and 6.40%. Conclusion The method is simple,sensitive,rapid, reliable and well reproducible,it can be used for the determination of five sweeteners in food simultaneously.
