Abstract:In this manuscript,water soluble polysaccharides were extracted from Tilia amurensis Rupr. flowers by microwave-assisted method was developed. The effect of microwave time,microwave power and ratio of raw material to liquid on the yield of polysaccharides was investigated and the microwave-assisted extraction process was optimized by response surface method. According to quadratic regression model,the optimum extraction parameters was 9 min,585 W and 1∶67(g∶mL). Under this optimum conditions,the yield of polysaccharides in theory was achieved at 12.57%.The physical and chemical properties experiment and infrared spectrum analysis indicated that polysaccharides extracted from Tilia amurensis Rupr. flowers presented typical properties of polysaccharides. The antioxidant activity in vitro of polysaccharides from Tilia amurensis Rupr. was evaluated by determining the reducing power,scavenging capacities on 1,1-diphenyl-2-picrylhydrazyl radical and hydroxyl free radical and the inhibition effect on lipid peroxidation induced by ferrous ion. The results showed that polysaccharides from Tilia amurensis Rupr. had certain reducing power,scavenging capacities on free radical and inhibition effect on lipid peroxidation,which were appreciably weaker than ascorbic acid(VC).
