Efficient Production of L-Sorbose by Gluconobacter oxydans

doi:10.3969/j.issn.1673-1689.2014.05.008

Home > Archive>Volume 33, Issue 5, 2014 >498-503. DOI:10.3969/j.issn.1673-1689.2014.05.008

Efficient Production of L-Sorbose by Gluconobacter oxydans
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                        10.3969/j.issn.1673-1689.2014.05.008
                    
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In order to achieve more efficient production of L-sorbose from D-sorbitol by Gluconobacter oxydans WSH-003, an engineered strain G. oxydans SLDH with overexpressed sorbitol dehydrogenase gene sldhAB was constructed. Effect of immobilized cells on a combination carrier of sodium alginate and diatomite to produce L-sorbose was investigated. Feeding strategies of immobilized fermentation were optimized in 1-L fermentor. A fed-batch fermentation model was established based on the kinetic model of D-sorbitol consumption in batch fermentation. By self-overexpressing the sldhAB gene in G. oxydans, the yields of L-sorbose increased by 20.3% while the fermentation time was shortened by 1/3 compared to the control strain in 1-L fermentor. Besides, the L-sorbose yield was raised by 30.3% using the fed-batch fermentation with a 19 days fermentation period in a bioreactor.

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WANG Xiaobei, ZHOU Jingwen, LIU Jie, DU Guocheng. Efficient Production of L-Sorbose by Gluconobacter oxydans[J]. Journal of Food Science and Biotechnology,2014,33(5):498-503.

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Online: October 19,2014
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