Abstract:The article has studied the inhibition effect of plant essential oil on common microorganisms in aquatic product. Method: Filter method is used to determine the bacteriostatic circle diameter of plant essential oil。As a result, three kinds of essential oils are selected. Then test-tube gradient dilution method is developed for the determination of three kinds of essential oil about bacteria minimal inhibitory concentration(MIC). Checkerboard dilution method is used for evaluating the joint bacteriostatic effect of oils by FICI value. Conclusion: Through the determination of bacteriostatic ring, cinnamon oil, Perilla oil and clove oil are selected. Each essential oil of antimicrobial MIC value range are: 0.313~0.625, 0.625~2.50, 0.625~2.50 μL/mL. Checkerboard dilution method results show that the distribution of essential oils for six antimicrobial showed synergistic and additive effect; there is no independent role and antagonism.