Biotransformation of 3-Cyanopyridine to Nicotinic Acid with Immobilized Pseudomonas putida CGMCC3830

doi:10.3969/j.issn.1673-1689.2014.08.003

Home > Archive>Volume 33, Issue 8, 2014 >800-804. DOI:10.3969/j.issn.1673-1689.2014.08.003

Biotransformation of 3-Cyanopyridine to Nicotinic Acid with Immobilized Pseudomonas putida CGMCC3830
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                        10.3969/j.issn.1673-1689.2014.08.003
                    
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Biotransformation of 3-cyanopyridine to nicotinic acid with immobilized Pseudomonas putida CGMCC3830 was studied. Sodium alginate was selected as the immobilization material. The cell immobilization conditions, biotransformation conditions and the batch stability were investigated. The optimized conditions for immobilization were as follows: sodium alginate 2 g/dL, calcium chloride 0.4 g/dL, curing time 6 h. The optimized immobilization conditions included: temperature 35 ℃, pH 7.0, substrate concentration 100 mmol/L. Results of batch conversion experiments showed that with the substrate concentration of 100 mmol/L, the nicotinic acid yield reached 91.8 g/g cell dry weight. The immobilized cell can be reused 10 times with the enzymatic activity retained 59.1%, while the free cell could be reused only 3 times with the enzymatic activity retained 45.6%.

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LI Heng, ZHAO Xin, YANG Tao, GONG Jinsong, ZHU Xiaoyan, XIONG Lei, LU Zhenming, XU Zhenghong, SHI Jinsong. Biotransformation of 3-Cyanopyridine to Nicotinic Acid with Immobilized Pseudomonas putida CGMCC3830[J]. Journal of Food Science and Biotechnology,2014,33(8):800-804.

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Online: October 19,2014
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