Diacylglycerol Kinase 3（CoDGK 3） Gene Cloning and Expresion Analysis of Camellia Oleifera

doi:10.3969/j.issn.1673-1689.2014.10.013

Home > Archive>Volume 33, Issue 10, 2014 >1090-1095. DOI:10.3969/j.issn.1673-1689.2014.10.013

Diacylglycerol Kinase 3（CoDGK 3） Gene Cloning and Expresion Analysis of Camellia Oleifera
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                        10.3969/j.issn.1673-1689.2014.10.013
                    
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Abstract:

Based on transcriptome Unigene sequence the diacylglycerol Kinase gene named CoDGK3（GeneBank accession number：KM068056） was isolated from Camellia oleifera with the method of RT-PCR and RACE. Bioinformatics approaches are used to analyze and predict the structural characters and physicochemical property. The bioinformatics analysis showed that the CDS of CoDGK3 is 1568 bp in length，OFR 1455 bp and it codes predicted protein of 485 amino acids. Homologous comparison and sequence analysis of CoDGK3 amino acids suggested that the CoDGK3 is highly conserved and showed the highest similarity（78%） with the DGK of Jatropha curcas and Populus trichocarpa.The gene expression changes by qPCR were similar to those of RTKM in transcriptome data indicate that the CoDGK3 expresses stably in different developmental stage of Camellia oleifera seeds and the transcriptome data of Camellia oleifera seeds is valid and reliable.

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JIANG Nan, ZHANG Weili, LI Jieyu, LIU Qilong, LIU Ming. Diacylglycerol Kinase 3（CoDGK 3） Gene Cloning and Expresion Analysis of Camellia Oleifera[J]. Journal of Food Science and Biotechnology,2014,33(10):1090-1095.

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Online: December 06,2014
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