Effect on Catalytic Activity and Conformation of Papain with Four Kinds of Organic Solvent

doi:10.3969/j.issn.1673-1689.2014.10.017

Home > Archive>Volume 33, Issue 10, 2014 >1112-1115. DOI:10.3969/j.issn.1673-1689.2014.10.017

Effect on Catalytic Activity and Conformation of Papain with Four Kinds of Organic Solvent
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                        10.3969/j.issn.1673-1689.2014.10.017
                    
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The alterations of papain activity and conformation were studied in four kinds of organic solvent，which are propanediol，glycerol，propanol and ethanediol. The result showed that the papain activity increased 20.1% in 15% propanediol，increased 17.8% in 8% propanol and decreased obviously in glycerol and ethanediol. The value of Km and Vmax was declined when treated with four kinds of organic solvent. Ultraviolet difference spectra and Fluorescence emission spectra showed that the secondary structure of papain altered in four kinds of organic solvent. The papain structure containning Trp residue was in a stronger non-polar environment in 40% glycerol，while the papain structure where coil and sheet increase contains little Trp residue in 15% propanediol and 10% ethanediol.

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CHU Zhizhan, CHEN Jipeng, LIU Xiaolin, HUANG Zhuolie, HUANG Lei. Effect on Catalytic Activity and Conformation of Papain with Four Kinds of Organic Solvent[J]. Journal of Food Science and Biotechnology,2014,33(10):1112-1115.

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Online: December 06,2014
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