Expression of D-Psicose 3-Epimerase in Bacillus subtilis

doi:10.3969/j.issn.1673-1689.2014.11.002

Home > Archive>Volume 33, Issue 11, 2014 >1129-1135. DOI:10.3969/j.issn.1673-1689.2014.11.002

Expression of D-Psicose 3-Epimerase in Bacillus subtilis
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                        10.3969/j.issn.1673-1689.2014.11.002
                    
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The D-psicose 3-epimerase gene was amplified through PCR and ligated with the expression vector pMA5 of Bacillus subtilis, forming the recombinant plasmid pMA5-cbdpe. The recombinant plasmid was then transformed into B. subtilis WB800, and a recombinant strain was acquired through resistance screening of Kanamycin and PCR identification method. The enzyme activity of recombinant D-psicose 3-epimerase could reach 6.8 U/mL after 18 h fermentation. The recombinant D-psicose 3-epimerase expressed in B. subtilis exhibited optimal activity at pH 7.0. And the optimal temperature was 55 ℃. It showed the same enzyme characterization as the D-psicose 3-epimerase expressed in Escherichia coli. This result indicated that the D-psicose 3-epimerase gene was successfully expressed in B. subtilis.

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JIA Min, MU Wanmeng, ZHANG Tao, JIANG Bo. Expression of D-Psicose 3-Epimerase in Bacillus subtilis[J]. Journal of Food Science and Biotechnology,2014,33(11):1129-1135.

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Online: December 19,2014
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