Recombinant Human Disintegrin Domain of ADAM15 Inhibits the Proliferation and Migration of HeLa Cells

doi:10.3969/j.issn.1673-1689.2014.11.004

Home > Archive>Volume 33, Issue 11, 2014 >1142-1147. DOI:10.3969/j.issn.1673-1689.2014.11.004

Recombinant Human Disintegrin Domain of ADAM15 Inhibits the Proliferation and Migration of HeLa Cells
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                        10.3969/j.issn.1673-1689.2014.11.004
                    
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The anti-proliferation and anti-migration activities of recombinant human disintegrin domain of ADAM15(rhddADAM15) were evaluated in vitro and in vivo and the related pathway was determined in this study. rhddADAM15 inhibited the proliferation and migration of human cervical cancer HeLa cells with the IC50 of 2.44 and 1.60 μmol/L respectively evaluated by SRB assay and wound healing assay. Over-expression of disintegrin domain of ADAM15 in HeLa cells by transient transfection induced the migration inhibition and partial S phase arrest. In addition, the inhibition effect of proliferation of HeLa cells aroused by rhddADAM15 was reversed by EGF, which is the agonist of MAPK signal pathways. Moreover, rhddADAM15 inhibited the growth and metastasis of HeLa cells xenograft in zebrafish. All the results showed that rhddADAM15 inhibited the proliferation and migration of HeLa cells by MAPK signal pathway and the target may exist both on the cell surface and in the cytoplasm.

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HOU Ying, CHU Min, CAI Yanfei, DU Fangfang, WANG Xiaomin, CHEN Yun, JIN Jian. Recombinant Human Disintegrin Domain of ADAM15 Inhibits the Proliferation and Migration of HeLa Cells[J]. Journal of Food Science and Biotechnology,2014,33(11):1142-1147.

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Online: December 19,2014
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